Part:BBa_K1058009:Experience

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Applications of BBa_K1058009

We prepared a series of bera-lactam solution of which the concentration was respectively 0μg/mL, 10μg/mL, 20μg/mL, 30μg/mL, 5μg/mL, 100μg/mL and 200μg/mL, then we add the solution to the bacteria liquid with BBa_K1058009 of which the OD is around 0.2~0.3 with the ratio of 1:1000 respectively, and the concentration of beta-lactam in the environment of the engineering E.coli in 8 different tubes is respectively 0ng/mL, 10ng/mL, 20ng/mL, 30ng/mL, 5ng/mL, 100ng/mL and 200ng/mL. The samples were taken to two 96-well plates once per hour or once per 30 minutes. The intensity of green fluorescence was tested with a fluorescence microplate reader. The results are as follows.

We constructed the beta-lactam sensor BBa_K1058009 successfully. The characterization data of the beta-lactam sensor showed a great linear relationship between the fluorescence intensity and the concentration of the beta-lactams to be detected in a certain range (0-200ng/mL for the beta-lactam sensor).

User Reviews

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